- The igblastn executable can now multi-thread much more efficiently for large sets of queries. The default number of threads is now four, but can be changed with the -num_threads option.
- The igblastn executable can now take an SRA accession as the query input. The search runs on the local machine, but the queries are retrieved from the SRA repository at the NCBI. Use the -sra rather than the -query option to enable.
- A lower default nucleotide mismatch penalty values for finding D and J genes (from -4 to -2 and from -3 to -2, respectively). This improves accuracy in finding the best D and J gene hits for moderately mutated sequences.
Our web IgBLAST page also uses the new default nucleotide mismatch penalty values (i.e., -2 for finding both D and J genes).
IgBLAST facilitates the analysis of immunoglobulin and T cell receptor variable domain sequences.