New in ClinVar – notifications for changes in the clinical interpretation of variants


We have added a new feature to ClinVar that allows you to follow a particular variant and be notified if the overall clinical interpretation in ClinVar changes, for example from a pathogenic category to a non-pathogenic one.  This service will let you know about changes that may require you to update your analysis reports and contact your patients and ordering physicians. The new feature allows you to follow a variant from the  variation page (Figure 1).  Simply click the “Follow” button to begin receiving notifications.

FollowFigure 1. A ClinVar variant page (VCV000541155.1) showing the ‘Follow’ button. The text on the button changes to ‘Following’ after you add  it to your followed variants. Clicking ‘Following’ presents the option to ‘Unfollow’, which removes the variant from the followed list when clicked.

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RefSeq Release 98 is public


RefSeq release 98 is accessible online, via FTP and through NCBI’s Entrez programming utilities, E-utilities.

This full release incorporates genomic, transcript, and protein data available as of January 6, 2020, and contains 223,560,051 records, including 161,133,441 proteins, 29,134,515 RNAs, and sequences from 98,406 organisms.

The release is provided in several directories as a complete dataset and as divided by logical groupings.

Read on for several important announcements.

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NCBI on YouTube: Get the most out of NCBI resources with these videos


Check out the latest videos on YouTube to learn how to best use NCBI graphical viewers, SRA, PGAP, and other resources.

Genome Data Viewer: Analyzing Remote BAM Alignment Files and Other Tips

This video shows you how to upload remote BAM files, and succinctly demonstrates handy viewer settings, such as Pileup display options, and highlights the very helpful tooltips in the Genome Data Viewer (GDV). There’s also a brief blog post on the same topic.

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Novel coronavirus complete genome from the Wuhan outbreak now available in GenBank


The complete annotated genome sequence of the novel coronavirus associated with the outbreak of pneumonia in Wuhan, China is now available from GenBank for free and easy access by the global biomedical community. Figure 1 shows the relationship of the Wuhan virus to selected coronaviruses.

Wuhan-human-1_posterior-output2

Figure 1.  Phylogenetic tree showing the relationship of Wuhan-Hu-1 (circled in red) to selected coronaviruses. Nucleotide alignment was done with MUSCLE 3.8. The phylogenetic tree was estimated with MrBayes 3.2.6 with parameters for GTR+g+i.  The scale bar indicates estimated substitutions per site, and all branch support values are 99.3% or higher.

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The new NIHMS system launch is just around the corner


Mark your calendars! As announced last month, a new NIH Manuscript Submission (NIHMS) system is coming! We are now pleased to share that the release is scheduled for January 23, 2020. To facilitate the transition, the NIHMS system will be temporarily unavailable beginning January 21.

Researchers are encouraged to take this schedule into consideration when preparing progress reports (e.g., RPPRs) and in completing other public access compliance activities. Papers deposited to NIHMS prior to January 21 will be migrated to the new system and any action(s) required after the release will be taken in the new system. Researchers will have access to information on all submissions made over time upon logging in to the new NIHMS.

Researchers with RPPRs due in February should address any outstanding compliance issues as soon as possible to avoid delays.

Discover what will be new at https://youtu.be/t2jvB1Oh51U.

Recent improvements to the genome Assembly resource


We’re constantly making improvements to the NCBI genome Assembly resource. This post points out some recent advances, highlighted in Figure 1 and described in more detail below.Surv_projFigure 1.  New improvements to the Assembly web pages. The results page showing the surveillance project filter (lower left), which excludes 28,220 Klebsiella pneumoniae assemblies from the Pathogen Detection Project, and the Download Assemblies button with a link to the File type description (circled in red, upper right). For other improvements in the Download Assemblies menu see our recent post.

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View BAM alignments in the NCBI genome browsers and sequence viewers sorted by haplotype tag


NCBI’s genome browsers and graphical sequence viewers now allow you to view BAM alignments sorted by haplotype tag. This option is useful for analyzing variants within a sequenced sample and can help you detect or validate structural variants.GDV_bamsFigure 1. Remote BAM alignment data sorted by haplotype tag in the Genome Data Viewer. The remote BAM file was added through the “User Data and Track Hubs” feature in GDV.  You can load the remote BAM for this example through https://go.usa.gov/xpM9c. The sorted display shows that haplotype 1 contains a significant deletion in this region relative to haplotype 2 and the reference genome assembly. Aligned reads not assigned a haplotype tag in the BAM file are grouped under the heading “haplotype not set” (not shown). 

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