Tag: Comparative Genomics Resource (CGR)

Re-evaluating the BLAST Nucleotide Database (nt)

Re-evaluating the BLAST Nucleotide Database (nt)

The ongoing sequencing revolution has resulted in exponential growth of the NCBI BLAST databases. The default BLAST nucleotide database (nt), the most popular Web BLAST database, is currently 903 billion letters and continues to grow rapidly – doubling in size in the last year. This growth will cause longer search times, reduced capacity, and more delays in updating the database. In the not-too-distant future, searching the entire nt database on the web will no longer be possible unless we modify the database scope and composition.

Because of the above concerns, we want to make the default Web BLAST nucleotide database smaller and more efficient. Some options are to:

    • Change its composition to improve the quality of sequence entries included
    • Take steps to slow its growth rate
    • Divide it into several databases by biological or functional categories

Continue reading “Re-evaluating the BLAST Nucleotide Database (nt)”

RefSeq Release 215

RefSeq Release 215

RefSeq release 215 is now available online, from the FTP site and through NCBI’s Entrez programming utilities, E-utilities.

This full release incorporates genomic, transcript, and protein data available as of November 7, 2022, and contains 335,372,031 records, including 244,583,657 proteins and sequences from 125,116 organisms. The release is provided in several directories as a complete dataset and also as divided by logical groupings. Continue reading “RefSeq Release 215”

New annotations in RefSeq!

New annotations in RefSeq!

In August and September, the NCBI Eukaryotic Genome Annotation Pipeline released thirty-eight new annotations in RefSeq for the following organisms:

  • Adelges cooleyi (spruce gall adelgid)
  • Aethina tumida (small hive beetle)
  • Anopheles aquasalis (mosquito)
  • Anopheles maculipalpis (mosquito)
  • Anthonomus grandis grandis (boll weevil)
  • Aphis gossypii (cotton aphid)
  • Bactrocera neohumeralis (fly)
  • Bombus affinis (bee)
  • Bombus huntii (bee)
  • Cataglyphis hispanica (ant)
  • Cygnus atratus (black swan) (pictured) Continue reading “New annotations in RefSeq!”
Now Available! Updated NCBI Datasets Command-Line Tools 

Now Available! Updated NCBI Datasets Command-Line Tools 

NLM’s NCBI Datasets announces the release of version 14 of our command-line (CLI) tools, datasets, and dataformat. This release (CLI v14.0.0) contains many improvements that are inspired by your feedback. It’s now easier than ever to browse and format metadata, generate customized tables, and download data packages. We hope these updates will improve your experience! 

NCBI Datasets CLIv14 includes changes to the command syntax, data package contents, and data report schemas that are not backwards-compatible. Commands written for CLI versions prior to version 14 may fail after the latest update. For more details see our FAQs.   Continue reading “Now Available! Updated NCBI Datasets Command-Line Tools “

Connect with NCBI at ASHG 2022

Connect with NCBI at ASHG 2022

Join us October 25-29 in Los Angeles, CA

We are looking forward to seeing you in-person at the American Society of Human Genetics (ASHG) annual meeting, October 25-29, 2022, in Los Angeles, California.

We will present a variety of talks and posters featuring our clinical and human genetic resources, as well as genome products and tools. We are excited to introduce the NIH Comparative Genomics Resource (CGR), a multi-year National Library of Medicine (NLM) project to maximize the impact of eukaryotic research organisms and their genomic data resources to biomedical research. If you’re interested in providing feedback that will be used to help drive CGR forward, consider joining our round table discussion.  

Check out NCBI’s schedule of activities and events: 

Continue reading “Connect with NCBI at ASHG 2022”

Coming soon! Changes to NCBI Datasets command-line tool in version 14 (CLIv14.0.0)

Coming soon! Changes to NCBI Datasets command-line tool in version 14 (CLIv14.0.0)

In October 2022, NCBI Datasets will release version 14 of our datasets and dataformat command-line tools. This release will contain breaking changes to the command syntax, content of the data packages and data reports. Thank you for your feedback that inspired these new features. We hope they will improve your experience!

We will continue to support CLI v13.x, although new features and improvements will be exclusive to CLI v14.0.0 release and up.

NCBI Datasets supports the NIH Comparative Genomics Resource (CGR), an NLM project to establish an ecosystem to facilitate reliable comparative genomics analyses for all eukaryotic organisms. Join our mailing list to keep up to date with NCBI Datasets and other CGR news.

More details

How is version 14 of the Datasets command-line tools (CLI v14.x) different from CLI v13.x and previous versions?  Continue reading “Coming soon! Changes to NCBI Datasets command-line tool in version 14 (CLIv14.0.0)”

Conserved Domain Database version 3.20 is available!

Conserved Domain Database version 3.20 is available!

A new version of the Conserved Domain Database (CDD) is now available. Version 3.20 contains 1,614 new or updated NCBI/CDD-curated domains and now mirrors Pfam version 34 as well as new models from the NCBIfam collection. Fine-grained classifications of the [(+)ssRNA] virus RNA-dependent RNA polymerase catalytic domain, RING-finger/U-box, dimerization/docking domains of the cAMP-dependent protein kinase regulatory subunit, and Galactose/rhamnose-binding lectin domain superfamily have been added, along with many other new models.

We have significantly increased the fraction of CD-Search and interactive BATCH CD-Search queries that yield results showing conserved domain architecture information and attributes that further characterize protein function through links to information-rich resources such as Enzyme Commission (EC) numbers , Gene Ontology (GO) terms, PubMed IDs, and identifiers from the CaZY, TCDB, and MEROPS databases. See our earlier post for additional details. You can access CDD and find updated content on the CDD FTP site at CDD version 3.20.

 Database statistics for CDD version 3.20:

Models Source
64,234 Total models from all Source Databases

Organized into 4,541 multi-model Superfamilies

18,882 NCBI CDD curation effort
1,125 NCBIfams
1,009 SMART v6.0
19,178 PFAM v34
4,871 COGs v1.0
10,140 NCBI Protein Clusters
4,488 TIGRFAM v15
59,693 Total models form the default CD-Search database

CD Search is part of the NIH Comparative Genomics Resource (CGR), an NLM project to establish an ecosystem to facilitate reliable comparative genomics analyses for all eukaryotic organisms.

Join our mailing list to keep up to date with CD Search and other CGR news.

RefSeq release 214 is available!

RefSeq release 214 is available!

RefSeq release 214 is now available online, from the FTP site, and through NCBI’s Entrez programming utilities, E-utilities.

This full release incorporates genomic, transcript, and protein data available as of September 12, 2022, and contains 328,588,569 records, including 239,609,016 proteins, 47,387,931 RNAs, and sequences from 123,394 organisms. The release is provided in several directories as a complete dataset and also as divided by logical groupings.

Foreign contamination screening
Introducing the new Foreign Contamination Screen (FCS) tool! If you produce assembled genomes, check out FCS, a tool you can run yourself to improve your genome assemblies and facilitate high-quality data submissions to GenBank. FCS is part of the NIH Comparative Genomics Resource (CGR), an NLM project to establish an ecosystem to facilitate reliable comparative genomics analyses for all eukaryotic organisms. See our previous blog post to learn how FCS enhances contaminant detection sensitivity. Continue reading “RefSeq release 214 is available!”

Join NCBI virtually at the Biodiversity Genomics 2022 conference

Join NCBI virtually at the Biodiversity Genomics 2022 conference

Learn about the NIH Comparative Genomics Resource (CGR) Project

The Biodiversity Genomics conference will take place virtually, October 2-7, 2022. This event is hosted by the Earth BioGenome Project and is open and free for all to attend.

NCBI staff will present a variety of recorded talks and posters highlighting various elements of the NIH Comparative Genomics Resource (CGR), including NCBI Datasets and the Comparative Genome Viewer (CGV). CGR is a multi-year National Library of Medicine (NLM) project to maximize the impact of eukaryotic research organisms and their genomic data resources to biomedical research. NCBI is charged with leading CGR development and engaging genomics communities. The CGR project will facilitate reliable comparative genomics analyses for all eukaryotic organisms in collaboration with the genomics community.

Check out NCBI’s schedule of activities to learn more about CGR: Continue reading “Join NCBI virtually at the Biodiversity Genomics 2022 conference”

Announcing new links and annotations on Conserved Domain Search results!

Announcing new links and annotations on Conserved Domain Search results!

Conserved Domain Search (CD Search) results now show domain architecture information and other annotations that further characterize predicted domain and protein function. These include links to PubMed, Gene Ontology (GO) terms, Enzyme Commission (EC) numbers, and the SPARCLE Domain Architecture Viewer. You can use these links on the results to find literature (PubMed), assign biological roles and protein function (GO and EC), and find proteins with the same domain architecture (Domain Architecture Viewer).  These annotations are currently available for a limited number of architectures, but we will continue to add them  as part of our curation effort.

Figure 1 shows the results of an example CD Search showing these new links.  Note that you can use the GO and EC information provided to retrieve protein models with these annotations from the Protein Family Models database, for example GO:0030246[GOTermId] — molecular function carbohydrate binding or  2.7.11.1[ECNumber]non-specific serine/threonine protein kinase.

Figure 1. Conserved Domain Database search results for a hypothetical protein (XP_007132600.1) from the common bean (Phaseolus vulgaris). The results classify the protein as a plant receptor-like protein kinase. The results also show the EC number and the GO terms associated with this domain architecture, a link to a PubMed citation for the protein family (receptor-like protein kinases), and a link to the Domain Architecture Viewer for G-type lectin S-receptor-like serine/threonine-protein kinases. The Domain Architecture Viewer shows other proteins from the NCBI databases with the same domain architecture (order, number and types of domains).  Continue reading “Announcing new links and annotations on Conserved Domain Search results!”