We’ve added a new feature (Max 3′ match), shown in Figure 1, to Primer-BLAST that limits the length of 3′ exon matches when designing exon-exon spanning primers. This makes it less likely that primers specifically designed to amplify transcripts will also amplify genomic DNA contamination in expression assays.

Figure 1. The new “Max 3′ match” option that limits the size of the 3′ match for exon-exon junction primers. This option helps avoid primers that may also produce product from genomic DNA. Continue reading “New feature added to Primer-BLAST to better design primers for expression assays” →