IgBLAST is a popular NCBI package for classifying and analyzing immunoglobulin (IG) and T cell receptor (TCR) variable domain sequences. We’ve released a new version (1.15.0) of IgBLAST with four new improvements / bug fixes:
- Support for the new framework region 4 (FWR4) annotation feature in the standard alignment formats and AIRR format.
- Renamed the previous “-penalty” parameter to -V_penalty to be consistent with other IgBLAST penalty options.
- Restored constant internal BLAST search parameters for domain annotation (i.e., FWR/CDR) so that this process is not influenced by user-provided parameters.
- Corrected FWR/CDR annotations for certain mouse VK and rat VH germline genes.
IgBLAST 1.15 is available for download from the BLAST FTP area. See the manual on GitHub for information about setting up and running IgBLAST.
IgBLAST 1.9.0 supports the adaptive immune receptor repertoire (AIRR) standard for sequence analysis results. The AIRR format is available on web IgBLAST as well as in the standalone IgBLAST tool, with the -outfmt 19 option.
Supporting this new schema in IgBLAST will enhance the increasing amount of repertoire studies that use next-generation sequencing technology to generate very large sets of Ig/T-cell receptor rearrangement analysis data.
IgBLAST facilitates the analysis of immunoglobulin and T cell receptor variable domain sequences. Get IgBLAST on FTP. A new manual is on GitHub.
IgBLAST 1.7.0 release
A new version of IgBLAST is now available on FTP, with the following new features:
- Specify whether overlapping nucleotides at VDJ junctions are allowed in matching V, D, and J genes.
- Set a custom J gene mismatch penalty
- Report the CDR3 start and stop positions in the sub-region table
- Use alignment length instead of percent identity as the tie-breaker for hits with identical blast scores, improving accuracy in the V, D, J gene assignment.
IgBLAST was developed at the NCBI to facilitate the analysis of immunoglobulin and T cell receptor variable domain sequences.