Tag: Multiple Sequence Alignment Viewer (MSAV)

Upcoming Changes to Virus Data Resources at NCBI

Effective June 2024, NCBI Virus will replace legacy virus web resources

Coming soon! As part of our ongoing effort to enhance your experience and modernize our services, several of our legacy virus-related web resources will be replaced by NCBI Virus – our community portal for viral sequence data. NCBI Virus is more comprehensive, modernized, and has more powerful features and analysis tools than our legacy resources.

What will change?

Below is a list of the legacy virus resources that will be replaced by NCBI Virus. The list includes a description of features that will continue to be supported through NCBI Virus: Continue reading “Upcoming Changes to Virus Data Resources at NCBI” →

Comparing Yeast Species Used in Beer Brewing and Bread Making

Using the NIH Comparative Genomics Resource (CGR) to gain knowledge about less-researched organisms

The scientific community relies heavily on model organism research to gain knowledge and make discoveries. However, focusing solely on these species misses valuable variation. Comparative genomics allows us to use knowledge from a model species, such as Saccharomyces cerevisiae, to understand traits in other, related organisms, such as Saccharomyces pastorianus or Saccharomyces eubayanus. Applying this information may provide valuable insight for other less-researched organisms. The National Institutes of Health (NIH) Comparative Genomics Resource (CGR) offers a cutting-edge NCBI toolkit of high-quality genomics data and tools to help you do just that. Continue reading “Comparing Yeast Species Used in Beer Brewing and Bread Making” →

Revolutionize your research with the NIH Comparative Genomics Resource (CGR)

Unlock the full potential of eukaryotic research organisms and their genomic data with the National Institutes of Health (NIH) Comparative Genomics Resource (CGR). CGR facilitates reliable comparative genomics analyses through community collaboration as well as an NCBI toolkit of interconnected, interoperable data and tools.  

Comparative genomics is a field of study that uses the genomes of many different organisms to help us understand basic biological processes and human disease. NCBI is developing CGR to help researchers take full advantage of the rapidly growing number of eukaryotic organisms that, due to recent technological advances, now have sequenced genomes and associated data that can be used in these types of studies. Its NCBI toolkit offers new and modern resources for such analyses, and its emphasis on community collaboration brings new opportunities to share and connect data. Continue reading “Revolutionize your research with the NIH Comparative Genomics Resource (CGR)” →

Connect with NCBI at ASHG 2022

Join us October 25-29 in Los Angeles, CA

We are looking forward to seeing you in-person at the American Society of Human Genetics (ASHG) annual meeting, October 25-29, 2022, in Los Angeles, California.

We will present a variety of talks and posters featuring our clinical and human genetic resources, as well as genome products and tools. We are excited to introduce the NIH Comparative Genomics Resource (CGR), a multi-year National Library of Medicine (NLM) project to maximize the impact of eukaryotic research organisms and their genomic data resources to biomedical research. If you’re interested in providing feedback that will be used to help drive CGR forward, consider joining our round table discussion.

Check out NCBI’s schedule of activities and events:

Continue reading “Connect with NCBI at ASHG 2022” →

New feature in the MSA viewer: Search for a short sequence

We’re reading and incorporating your feedback! As requested, you can now search for sequences in our Multiple Sequence Alignment (MSA) Viewer. You can search the anchor or consensus sequence of a multiple alignment for short sequence strings. This new feature allows you to:

- Look for a sequence motif in DNA or protein alignments in order to confirm the presence of a probe or PCR primer.
- Check whether your sequence has matches in multiple locations on the anchor or consensus.
- Examine the sequence similarity within the alignment of your match. Continue reading “New feature in the MSA viewer: Search for a short sequence” →

NCBI on YouTube: Customize MSA Viewer, SciENcv, plants and RNA-Seq data, Datasets and PubMed

Missed a few videos on YouTube? Here’s the latest from our channel.

Customize the MSA Viewer to Make Your Analysis Easier

We’re constantly improving the Multiple Sequence Alignment (MSA) Viewer. This video demonstrates several new and popular features, including the ability to change data columns, hide selected rows, analyze polymorphisms, and more.

Continue reading “NCBI on YouTube: Customize MSA Viewer, SciENcv, plants and RNA-Seq data, Datasets and PubMed” →

Customize columns in NCBI’s Multiple Sequence Alignment Viewer

We’re excited to report that researchers using the NCBI Multiple Sequence Alignment Viewer (MSAV) can now add or remove columns from the alignment view. In this way, you can choose to show only columns with data relevant for analysis of the sequences in your alignment.

When you arrive at an MSA alignment view, you’ll see columns for the Sequence ID (e.g., sequence accession number), Start and End of the alignment, and the organism (species name).

Sometimes, the information in these default columns isn’t the most useful information for sorting through the alignment. In the example above, all the sequences are from the same organism, so looking at the Organism column won’t help in figuring out the differences among the different sequences in the alignment.

Continue reading “Customize columns in NCBI’s Multiple Sequence Alignment Viewer” →

Hiding sequences in an alignment now available in the MSA Viewer!

Do you ever wish there was a quick way to hide partial or poor quality sequences from a multiple alignment view? NCBI’s Multiple Sequence Alignment Viewer (MSAV) now allows you to do just that with an easy hide/show rows feature! Hidden rows won’t be shown in the PDF/SVG download, and these sequences will not be included in the FASTA alignment download file.

You can easily manage which rows are shown through the menu available by right-clicking on a row or through the Rows dialog (Figure 1).

Figure 1. The MSA viewer showing the options for hiding or showing rows. Right clicking any sequence row provides options for hiding single or selected rows or restoring hidden rows. You can also manage rows through the edit row dialog activated by clicking the “Rows” button next to the gear icon at the upper-right. Check or uncheck sequences to add or remove them from the display. The “Rows shown” status message at the lower-right of the MSAV indicates the total number of rows in the sequence alignment and the number displayed.

Keep in mind that hiding rows does not re-calculate the alignment, so it’s important to know if any rows have been hidden from your current view. The “Rows shown” message at the lower-right indicates whether you are displaying all rows.

You can find more tips on using the MSA Viewer, including information about anchors, consensus, and coloring settings in our user guide. Please get in touch if you have any questions or suggestions using the Feedback link on the page or writing to the NCBI Help Desk.

Recent enhancements in Genome Workbench version 3.4.1

New Features

Version 3.4.1 of Genome Workbench, NCBI’s sequence annotation and analysis platform, includes new features for the Multiple Sequence Alignment View, the Graphical Sequence View and the Sequence Editing and Submission Package as well as a number of other improvements and bug fixes.

In the Multiple Sequence Alignment View, you can now export publication quality graphics (Save As PDF/SVG … , Figure 1). In the Graphical Sequence View you can now search by locus tag, use improved search capabilities for genes by locus and can better display the selected location in the feature editing dialog when annotating a sequence.

MSA Figure 1. A multiple alignment view in Genome Workbench highlighting the new ability to save presentation quality image files (Save As PDF and SVG formats).

In the Sequence Editing and Submission Package, we rearranged the controls in the Table Reader dialog to fit onto smaller screens and improved importing feature tables that contain mat-peptides (mature peptide) features.

Bug Fixes and Improvements

We have made a number of other fixes and improvements. For MacOS users we fixed blurry text in some dialogs, fixed the copy to clipboard problem, and improved support for the latest Catalina version. We also fixed a crashing problem in the Active Object Inspector interface. You should also see improvements in loading SNP data and better recovery in cases of power outages or other events causing local file corruption.

In the Sequence Editing and Submission Package, we fixed a bug that occurred when applying miscellaneous descriptors and structured comment fields using the Table Reader and an issue with using a PubMed ID to look up a publication.

Please see the extensive help documentation including FAQs, videos, and tutorials linked to the Genome Workbench homepage for more information and examples on how to use Genome Workbench in your research.

Download high-quality graphics from the NCBI Multiple Sequence Alignment Viewer (MSAV)

You can now download a publication-quality graphic images of the alignment displayed in the NCBI Multiple Sequence Alignment Viewer (Figure 1). Load sequence alignments into the viewer from BLAST or COBALT results or upload alignment files directly. Once you have the the alignment set in the viewer, choose the “Printer-friendly PDF/SVG” option in the Download menu on the toolbar to save the image. The PDF and SVG files contain vector graphics suitable for presentation and publication. MSA_download Figure 1. The image download options in the MSAV. You can adjust the desired coordinate range and choose to download a PDF or SVG image. You can also preview the PDF download . Choose simplified color shading to improve compatibility with some graphics programs.

The downloaded image will show the coordinate range you requested and will include all the rows in the alignment.

Please contact us through the Feedback link on the MSA Viewer or write to the NCBI Help Desk to provide feedback and let us know how we can make the NCBI Multiple Sequence Viewer work better for you.